Existing ICSI has a limitation in screening morphologically perfect sperm since it performs fertilization on sperm magnified 200~400 times, but we obtain high fertilization and pregnancy rates by using special high-resolution equipment to the case of failed fertilization in a general micromanipulation procedure or patient with a low fertilization rate.

Maria Fertility Hospital has recently been using super-precision laser, which is widely used for medical procedures, in assisted hatching. The principle of laser-assisted hatching is similar to the use of lasers in sight correction procedures (LASIK, LASEK, etc.) which are well-known ophthalmic procedures. In other words, this is a technique that shaves off some of the zona pellucida that surrounds the ovum with a laser and thus helps the embryo burst through more easily.

Assisted hatching using a laser is the latest technique that can increase pregnancy and implantation rates by minimizing harmful factors for embryos compared to the existing assisted hatching that was used in IVF centers and providing a more stable environment for embryos and helping hatching by shortening the duration of the procedure.

· When the zona pellucida of the embryo is thick
· Women aged over 38 who undergo IVF
· When IVF fails repeatedly
· When the amount of follicle stimulating hormone (FSH) in blood is high

In the past, the FISH method was used when genetic testing was conducted by using a fluorescent probe on specific chromosomes. However, this has a limitation of testing only selected chromosomes. Recently, PGS, which can examine any abnormality in all chromosomes, was developed in Europe and the U.S.

Currently, the subfertility medical research division of Maria Fertility Hospital is making efforts for healthy babies by performing research and development on PGS and applying it in a clinical setting.

PGD (Preimplantation Genetic Diagnosis)

PGD is a technique that performs in vitro fertilization of ovum and sperm, picks one or two blastomeres from an eight-cell stage embryo or blastocyst-stage embryo, and conducts chromosome examination of genetic testing to screen for a normal fertilized egg and then transfers and implants it for pregnancy.

PGS (Preimplantation Genetic Screening)

Although this is the same as PGD in that it takes part of a fertilized embryo to conduct a genetic test, it examines a total of 46 chromosomes to see if there are numerical chromosome aberrations, damage or amplification using array CGH technology. This is a more sensitive and accurate testing method.

• http://www.ivf1.com
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In the results of a preliminary experiment using an experimental mouse embryo, the new device showed more excellent results in both mitotic phase embryo and blastocyst-stage embryo than existing devices and it was confirmed to be appropriate for oocyte banking, the need for which is growing. We published this in a medical journal.